38.6 B12

38.6.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

PRL (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 19.1588240 1 <0.0001 ***
B12 1.7685130 1 0.2 ns
Study 1.3894630 1 0.2 ns
GA_samp 20.5955284 1 <0.0001 ***
B12:Study 0.9997984 1 0.3 ns
hPL (μg/ml)
Chisq Df Pr(>Chisq)
(Intercept) 0.2575183 1 0.6 ns
B12 3.3000490 1 0.07 ns
Study 8.2723140 1 0.004 **
GA_samp 333.6759676 1 <0.0001 ***
B12:Study 4.0852948 1 0.04 *
GH2 (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 34.4484224 1 <0.0001 ***
B12 1.2840040 1 0.3 ns
Study 0.7046147 1 0.4 ns
GA_samp 114.5064198 1 <0.0001 ***
B12:Study 0.7066501 1 0.4 ns
Insulin (uU/ml)
model term df1 df2 F.ratio Chisq p.value
1 B12 1 Inf 3.634 3.634 0.06 ns
3 Study 1 Inf 2.684 2.684 0.1 ns
4 GA_samp 1 Inf 0.706 0.706 0.4 ns
2 B12:Study 1 Inf 0.973 0.973 0.3 ns
HOMA-IR
model term df1 df2 F.ratio Chisq p.value
1 B12 1 Inf 2.725 2.725 0.10 ns
3 Study 1 Inf 3.060 3.060 0.08 ns
4 GA_samp 1 Inf 0.292 0.292 0.6 ns
2 B12:Study 1 Inf 0.561 0.561 0.5 ns
Glucose (mmol/L)
model term df1 df2 F.ratio Chisq p.value
1 B12 1 Inf 0.050 0.050 0.8 ns
3 Study 1 Inf 3.602 3.602 0.06 ns
4 GA_samp 1 Inf 0.243 0.243 0.6 ns
2 B12:Study 1 Inf 0.009 0.009 0.9 ns

38.6.2 Model summary

Ratio of Geometric means (95% CI)

PRL (ng/ml)
Var Effect P
(Intercept) 14.07 (5.55, 35.49) <0.0001
B12 1.00 (1.00, 1.00) 0.25
StudySTOP 1.11 (0.81, 1.52) 0.53
GA_samp 1.13 (1.07, 1.20) <0.0001
hPL (μg/ml)
Var Effect P
(Intercept) 1.17 (0.64, 2.12) 0.61
B12 1.00 (1.00, 1.00) 0.07
StudySTOP 1.74 (1.20, 2.55) 0.005
GA_samp 1.34 (1.30, 1.39) <0.0001
B12:StudySTOP 1.00 (1.00, 1.00) 0.05
GH2 (ng/ml)
Var Effect P
(Intercept) 0.11 (0.06, 0.22) <0.0001
B12 1.00 (1.00, 1.00) 0.31
StudySTOP 1.02 (0.81, 1.29) 0.85
GA_samp 1.24 (1.19, 1.29) <0.0001
Insulin (uU/ml)
Var Effect P
(Intercept) 24.45 (6.43, 92.92) <0.0001
B12 1.00 (1.00, 1.00) 0.07
StudySTOP 0.65 (0.41, 1.01) 0.06
GA_samp 1.03 (0.95, 1.12) 0.41
HOMA-IR
Var Effect P
(Intercept) 7.57 (1.79, 31.93) 0.006
B12 1.00 (1.00, 1.00) 0.08
StudySTOP 0.51 (0.32, 0.83) 0.007
GA_samp 1.02 (0.94, 1.12) 0.60
Glucose (mmol/L)
Var Effect P
(Intercept) 6.66 (5.00, 8.88) <0.0001
B12 1.00 (1.00, 1.00) 0.77
StudySTOP 0.79 (0.73, 0.86) <0.0001
GA_samp 0.99 (0.97, 1.02) 0.63

38.6.3 Estimated means

Estimated marginal means (or adjusted means) adjusted for GA at sampling.

Results are averaged over the levels of B12 concentration (pM) and may be misleading when interaction term is involved.

PRL (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 73.72646 15.24405 52.80949 48.69659 111.6216
STOP 81.60376 13.44571 23.17142 58.04193 114.7304
hPL (μg/ml)
Study response SE df lower.CL upper.CL
SCOPE 63.46718 5.128348 107.624 54.07385 74.49225
STOP 73.77854 3.062191 31.805 67.79608 80.28891
GH2 (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 1.767604 0.1832952 133.77534 1.439834 2.16999
STOP 1.809009 0.0935599 40.47779 1.629527 2.00826
Insulin (uU/ml)
Study response SE df asymp.LCL asymp.UCL
SCOPE 27.59461 5.329078 Inf 18.89905 40.29105
STOP 17.86612 1.409637 Inf 15.30630 20.85403
HOMA-IR
Study response SE df asymp.LCL asymp.UCL
SCOPE 7.467598 1.5526156 Inf 4.968262 11.224251
STOP 3.835829 0.3258385 Inf 3.247529 4.530702
Glucose (mmol/L)
Study response SE df asymp.LCL asymp.UCL
SCOPE 6.125908 0.2464818 Inf 5.661370 6.628563
STOP 4.843695 0.1053213 Inf 4.641606 5.054583

38.6.4 Comparisons

Effect for every 100 unit increase in B12 concentration (pM).
Hormone Contrast Study Ratio of Geometric means (95% CI) P-value
PRL (ng/ml) B12376.5 / B12276.5 SCOPE 1.04 (0.97, 1.11) 0.2
PRL (ng/ml) B12376.5 / B12276.5 STOP 1.04 (0.97, 1.11) 0.2
hPL (μg/ml) B12376.5 / B12276.5 SCOPE 1.11 (0.99, 1.24) 0.07
hPL (μg/ml) B12376.5 / B12276.5 STOP 0.98 (0.94, 1.02) 0.4
GH2 (ng/ml) B12376.5 / B12276.5 SCOPE 1.03 (0.97, 1.08) 0.3
GH2 (ng/ml) B12376.5 / B12276.5 STOP 1.03 (0.97, 1.08) 0.3
Insulin (uU/ml) B12376.5 / B12276.5 SCOPE 0.92 (0.83, 1.01) 0.07
Insulin (uU/ml) B12376.5 / B12276.5 STOP 0.92 (0.83, 1.01) 0.07
HOMA-IR B12376.5 / B12276.5 SCOPE 0.91 (0.82, 1.01) 0.08
HOMA-IR B12376.5 / B12276.5 STOP 0.91 (0.82, 1.01) 0.08
Glucose (mmol/L) B12376.5 / B12276.5 SCOPE 1.00 (0.97, 1.02) 0.8
Glucose (mmol/L) B12376.5 / B12276.5 STOP 1.00 (0.97, 1.02) 0.8

38.6.5 Estimated means (50 unit increase)

Estimated marginal means (or adjusted means) adjusted for GA at sampling.

Results are averaged over the levels of B12 concentration (pM) and may be misleading when interaction term is involved.

PRL (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 73.00664 15.06560 52.44310 48.25712 110.4494
STOP 80.80704 13.28582 22.96874 57.50787 113.5458
hPL (μg/ml)
Study response SE df lower.CL upper.CL
SCOPE 61.84483 4.770209 108.14783 53.07691 72.06114
STOP 74.15276 3.026155 29.75089 68.22106 80.60020
GH2 (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 1.755476 0.1818003 131.10262 1.430282 2.154608
STOP 1.796597 0.0911438 38.16912 1.621269 1.990885
Insulin (uU/ml)
Study response SE df asymp.LCL asymp.UCL
SCOPE 28.21420 5.465730 Inf 19.30059 41.24438
STOP 18.26727 1.412773 Inf 15.69793 21.25713
HOMA-IR
Study response SE df asymp.LCL asymp.UCL
SCOPE 7.640793 1.5968818 Inf 5.072736 11.508922
STOP 3.924793 0.3272153 Inf 3.333120 4.621496
Glucose (mmol/L)
Study response SE df asymp.LCL asymp.UCL
SCOPE 6.131210 0.2501140 Inf 5.660081 6.641555
STOP 4.847888 0.1041656 Inf 4.647966 5.056408

38.6.6 Comparisons (50 unit increase)

Effect for every 50 unit increase in B12 concentration (pM).
Hormone Contrast Study Ratio of Geometric means (95% CI) P-value
PRL (ng/ml) B12326.5 / B12276.5 SCOPE 1.02 (0.99, 1.05) 0.2
PRL (ng/ml) B12326.5 / B12276.5 STOP 1.02 (0.99, 1.05) 0.2
hPL (μg/ml) B12326.5 / B12276.5 SCOPE 1.05 (1.00, 1.11) 0.07
hPL (μg/ml) B12326.5 / B12276.5 STOP 0.99 (0.97, 1.01) 0.4
GH2 (ng/ml) B12326.5 / B12276.5 SCOPE 1.01 (0.99, 1.04) 0.3
GH2 (ng/ml) B12326.5 / B12276.5 STOP 1.01 (0.99, 1.04) 0.3
Insulin (uU/ml) B12326.5 / B12276.5 SCOPE 0.96 (0.91, 1.00) 0.07
Insulin (uU/ml) B12326.5 / B12276.5 STOP 0.96 (0.91, 1.00) 0.07
HOMA-IR B12326.5 / B12276.5 SCOPE 0.96 (0.91, 1.01) 0.08
HOMA-IR B12326.5 / B12276.5 STOP 0.96 (0.91, 1.01) 0.08
Glucose (mmol/L) B12326.5 / B12276.5 SCOPE 1.00 (0.99, 1.01) 0.8
Glucose (mmol/L) B12326.5 / B12276.5 STOP 1.00 (0.99, 1.01) 0.8

38.6.7 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

38.6.8 Plots